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Fig. 1 | Genome Biology

Fig. 1

From: Single-cell transcriptome profiling of an adult human cell atlas of 15 major organs

Fig. 1

Overview of single-cell RNA sequencing of 15 organ tissues from a male adult donor. a An experiment schematic diagram highlighting the sites of the organs for tissue collection and sample processing. Live cells were collected using flow cytometry sorting (FACS) and subjected for cell barcoding. cDNA libraries for TCR, BCR, and 5′-mRNA expression were constructed independently, followed by high-throughput sequencing and downstream analyses. b t-SNE visualization of all cells (84,363) in organs. Each dot represents one cell, with colors coded according to the origin of organ. Labeled cell types are the predominant cell types in each cluster. c Dot plots showing the most highly expressed marker genes (x-axis) of major cell types (y-axis) in b. The depth of the color from white to blue and the size of the dot represent the average expression from low to high and the percent of cells expressing the gene

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