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Fig. 3 | Genome Biology

Fig. 3

From: Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

Fig. 3

High confidence OTS-sites in HEK293 cells. Visualization of the 55 gRNA binding sites for ATXN10, MMP14, and NEK1 that were detected both by SMRT-OTS and Nano-OTS. The letters at the top show the gRNA sequence and the bases (NGG) that corresponds to the PAM site. Below are the on-target and all off-target sequences detected by the OTS methods. Colored letters correspond to single nucleotide mismatches in the HEK293 genome as compared with the gRNA sequence. Triangles and hyphens (-) are used to mark sites where nucleotides need to be inserted or deleted, respectively, in the HEK293 genome in order to match the gRNA sequence. The column to the right contains the combined read count from the SMRT-OTS and Nano-OTS assays, for each OTS-site

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