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Table 1 A summary of the studies on CRISPR-mediated plant disease resistance

From: Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants

Pathogen type Plant(s) Desired modification Targeted DNA/RNA Targeted pathogen(s)/disease(s) Results Reference
m Arabidopsis Virus RNA genome disruption Virus RNA genome Turnip mosaic virus Indels in virus RNA [69]
N. benthamiana Virus RNA genome disruption Virus RNA genome Turnip mosaic virus Indels in virus RNA [37]
Rice, N. benthamiana Virus RNA genome disruption Virus RNA genome Southern rice black-streaked dwarf virus, Tobacco mosaic virus Reduction in virus levels and disease symptoms [70]
Arabidopsis, N. benthamiana Virus RNA genome disruption Virus RNA genome Cucumber mosaic virus, Tobacco mosaic virus Reduction in virus levels and disease symptoms [71]
N. benthamiana Virus DNA disruption Virus DNA
Rep, IR, and Cp
Beet curly top virus, Merremia mosaic virus, Tomato yellow leaf curl virus Indels in virus DNA [72]
N. benthamiana Virus DNA disruption Virus DNA and satellite sequences Cotton leaf curl Kokhran virus, Tomato yellow leaf curl Sardinian virus, Tomato yellow leaf curl virus, Merremia mosaic virus, BCTV-Logan, BCTV-Worland Indels in virus DNA [73]
N. benthamiana Virus DNA disruption Virus DNA
Rep A/Rep and LIR
Bean yellow dwarf virus Indels in virus DNA, resistance to virus [74]
Arabidopsis, N. benthamiana Virus DNA disruption Virus DNA
Rep, IR, and CP
Beet severe curly top virus Indels in virus DNA, resistance to virus [75]
Tomato, N. benthamiana Virus DNA disruption Virus DNA
Rep, IR, and Cp
Tomato yellow leaf curl virus Indels in virus DNA, resistance to virus [76]
N. benthamiana Virus DNA disruption Multiplex editing at Rep and IR Cotton leaf curl Multan virus Significantly low virus accumulation and decreased disease symptoms [77]
Cassava Virus DNA disruption AC2 and AC3 African cassava mosaic virus Indels in virus DNA but no virus resistance [78]
N. benthamiana Virus DNA disruption Multiplex editing at virus DNA Rep, IR, and Cp Chilli leaf curl virus Significantly low virus accumulation and decreased disease symptoms [79]
Banana Virus DNA disruption Virus sequences in the host plantain genome Endogenous banana streak virus 75% of pl0ants remain asymptomatic [80]
  Biomimickinga Eif4e1 Clover yellow vein virus Reduced virus accumulation [65]
Rice Biomimickinga Eif4g Rice tungro spherical virus Resistance to virus [81]
Cassava Gene disruption nCBP-1, nCBP-2 Cassava brown streak disease Suppressed disease symptoms [82]
Arabidopsis Gene disruption EIF4E Turnip mosaic virus Resistance to virus [47]
Cucumber Gene disruption eIF4E Cucumber vein yellowing virus (ipomovirus), Zucchini yellow mosaic virus, and Papaya ring spot mosaic virus-W (potyviruses) Resistance to three viruses [46]
Fungus Tomato Gene disruption Multiplex gRNA at Pmr4 Powdery mildew caused by Oidium neolycopersici Significant reduction in mildew symptoms [83]
Tomato Gene disruption SlMapk3 Botrytis cinerea Increased resistance to B. cinerea [84]
Tomato Gene disruption Solyc08g075770 Fusarium wilt Tolerance to fusarium wilt [85]
Rice Gene disruption Single and multiplex gRNA at OsERF922 Rice blast caused by Magnaporthe oryzae Significantly decreased blast lesions [86]
Grape Gene disruption VvWRKY52 B. cinerea Increased resistance to B. cinerea [87]
Tomato Gene disruption SlMlo1 Powdery mildew Resistance to powdery mildew [88]
Banana Gene insertion RGA2, Ced9 Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (TR4) Significant reduction in disease [89]
Rice Gene disruption OsMPK5 Fungal (Magnaporthe grisea) and bacterial (Burkholderia glumae) pathogens Indels in the target; resistance not confirmed [90]
Grape Gene disruption Mlo-7 Powdery mildew Indels in the target; resistance not confirmed [91]
Wheat Gene disruption TaMlo-A1, TaMlo-B1, and TaMlo-D1 Powdery mildew High tolerance to powdery mildew [92]
Wheat Gene disruption TaMlo Powdery mildew Indels in the target; resistance not confirmed [30]
Wheat Gene disruption TaEdr1 (three homologs) Powdery mildew Resistance to powdery mildew [93]
Bacteria Rice Gene disruption OsSWEET13 Bacterial blight caused by Xanthomonas oryzae pv. Oryzae (Xoo) Resistance not confirmed [94]
Rice Gene disruption OsSWEET11 Bacterial blight Enhanced resistance to Xoo [95]
Rice Gene and promoter disruption TALE-binding elements (EBEs) in OsSWEET13 promoter, OsSWEETT11, and OsSWEEt14 genes Bacterial blight caused by Xoo Broad-spectrum resistance against multiple Xoo strains [50]
Rice Promoter disruption OsSWEET11, OsSWEET13, and OsSWEET14 Bacterial blight Increased resistance to bacterial blight; confirmed in field trials [51]
Apple Gene disruption DIPM-1, DIPM-2, and DIPM-4 Fire blight disease (caused by Erwinia amylovora) Indels in the target; resistance not confirmed [91]
Rice Promoter disruption OsSWEET11, OsSWEET14 Bacterial blight Indels in promoter; disease resistance not confirmed [96]
Tomato Gene repair Jaz2 Bacterial speck disease caused by Pseudomonas syringae pv. tomato DC 3000 Resistance to bacterial speck disease [97]
Tomato Gene disruption Dmr6 Pseudomonas syringae, Phytophthora capsici, and Xanthomonas spp. Resistance to P. syringae, P. capsici, and Xanthomonas spp. [98]
Grapefruit Promoter disruption CsLOB1 Citrus canker Significantly reduced canker symptoms [99]
Wanjincheng orange Promoter disruption CsLOB1 Citrus canker Disease severity decreased by 83.2–98.3% [100]
Oomycete Papaya Gene disruption PpalEPIC8 Phytophthora palmivora Increased resistance against P. palmivora [101]
Theobroma cacao Gene disruption TcNPR3 Phytophthora tropicalis Increased resistance against P. tropicalis [102]
  1. aBiomimicking refers here to the introduction of CRISPR-mediated mutations in such a way that the sequence of a target gene in disease-susceptible variety is converted to the sequence from a disease-resistant variety. Thus, instead of replacing the whole gene, the researcher introduces only the specific mutations associated with the disease resistance trait, assuming that the nucleotide differences between the gene of interest in the cultivated and wild varieties are not otherwise significant to plant viability and productivity