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Fig. 4 | Genome Biology

Fig. 4

From: Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription

Fig. 4

Colocalization of H3K9me3 and HP1α in U2OS cells. a Imaging of H3K9me3 and HP1α colocalization by widefield fluorescence microscopy. H3K9me3 puncta (green) were detected by H3K9me3 antibody and HP1α (magenta) was tagged by HaloTag. b Linescan of the vertical line. Linescan shows, from top to bottom, the intensities (arbitrary units) of H3K9me3 (green) and HP1α (magenta) along the line indicated in a. c Imaging of H3K9me3 and HP1α colocalization by widefield fluorescence microscopy 3D-SIM. The image processing details are the same as described in a. d Linescan of the vertical line. The image processing details are the same as described in b. e Percentage of overlapping area between HP1α and H3K9me3 from images captured by widefield fluorescence microscopy and 3D-SIM. The outer edges of the box are the 25th and 75th percentiles. The whiskers extend to the minimum and maximum values. n = 20 cells in each experiment

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