Skip to main content
Fig. 3 | Genome Biology

Fig. 3

From: Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription

Fig. 3

Tracking the Dynamics of C19Q loci in EpiGo-KRAB cells. a Live cell tracking of C19Q loci movement in EpiGo-Control cell lines. The dynamics of C19Q (green) and HP1α (magenta) were tracked for 30 s. The movement of locus C19Q loci (arrowed) were recorded at 100 ms per frame for 30 s. b All trajectories were shifted to start from the origin (0, 0) for easy comparison of the movement vectors. The locus movements were corrected for the possible movements of microscope stage. c Displacement of C19Q loci in EpiGo-Control cell lines. Distance to the start point of each time point was measured and plotted. n = 20 cells. d Live cell tracking of C19Q loci movement in EpiGo-KRAB cell lines. The image processing details are the same as described in a. e All trajectories were shifted to start from the origin (0, 0) for easy comparison of the movement vectors. The locus movements were corrected for the possible movements of microscope stage. f Displacement distance of C19Q loci in EpiGo-KRAB cell lines. Distance to the start point of each time point was measured and plotted. n = 17 cells

Back to article page