Fig. 1

A CRISPR-based EpiGo-KRAB system to track genome organization upon epigenetic modification. a A scheme of the EpiGo system. EpiGo (Epigenetic perturbation induced Genome organization) system consists of dCas9-KRAB, PCP-GFP, and sgRNA-2XPP7 targeting to C19Q repeats, which contain 836 copies of target sites on the q-arm of chromosome 19. C19Q-KRAB will recruit SETDB1 and induce H3K9 trimethylation at each target sites. H3K9me3 regions will recruit HP1α and induce genome organization. b CRISPR-based imaging of C19Q in presence of PCP-GFP, C19Q-sgRNA-2XPP7, and dCas9, marked with GFP-C19Q. The HP1α-HaloTag stable cell lines was used to examine the colocalization between C19Q (green) and HP1α (magenta). c CRISPR imaging with the same conditions as b except that dCas9 was replaced by dCas9-KRAB and marked with GFP-C19Q-KRAB. d H3K9me3 state of EpiGo-Control (dCas9) and EpiGo-KRAB (dCas9-KRAB) cell lines. ChIP-seq of H3K9me3 was performed in these two cell lines. C19Q-1 region (chr19: 48,800,000–52,300,000) is chosen to show the difference of H3K9me3 state between EpiGo-Control and EpiGo-KRAB cell lines