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Fig. 6 | Genome Biology

Fig. 6

From: Intronic tRNAs of mitochondrial origin regulate constitutive and alternative splicing

Fig. 6

NimtRNA protein interaction analysis. a Biotinylated transcripts containing or lacking nimtRNAs were incubated with nuclear protein extracts. Subsequently, binding proteins were isolated and analyzed by PAGE; differential band patterns were excised and analyzed by MS. b GO analysis of nimtRNA transcript interacting proteins. c The top 10 nimtRNA transcript interacting proteins ranked by absolute abundance. d–f An electrophoretic mobility shift assay was performed with increasing concentrations of KHDRBS1 being incubated with a transcript of the nimtRNATyr (d), nimtRNATyr delT (e), or snoRNA SNORD115 (f). The unbound transcript is indicated by an asterisk; the transcript-protein complexes are indicated by triangles

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