Fig. 3
From: A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila
Specificity evaluation of Drosophila codon-optimized Cas13 variants in Sg4 cells. a–f Relative expression levels of eCFP when using different Cas13 variants and crRNAs that carry a range of single mismatches along the eCFP-crRNA-2. Data were normalized to samples treated with a blank crRNA (control = C). eCFP expression levels in Cas13/ wild-type (WT) crRNA samples were also included as a reference. * = p value < 0.05, ** = p value < 0.01, *** = p value < 0.001, ns = not significant, p values based on Dunnett’s post hoc test, error bars represent 95% confidence intervals