Fig. 5

CMT3-dependent CHG methylation compensates pol2a-12 molecular defects. a Metaplots showing TE methylation rates in CHG context in the indicated genotypes. Annotations were aligned to their 5′ or 3′ end and average methylation was calculated for each 100-bp bin from 3 kb upstream to 3 kb downstream. b Transcript accumulation in reads per kilobase per million mapped reads (RPKM) at the indicated genes. Two replicates per samples are shown. c TE methylation changes in CHG context in pol2a-12 and pol2a-12 cmt3 normalized to WT and cmt3, respectively. d Venn diagrams showing the overlap between TEs upregulated in pol2a-12, cmt3 and pol2a-12 cmt3. e (left) Relative heterochromatic fraction (RHF) evaluated from DAPI-stained pictures of nuclei from the indicated genotypes. The effect of genotype was verified with a Kruskal-Wallis rank-sum test. Significant differences between groups were evaluated by a Dwass-Steel-Crichtlow-Fligner test and are indicated by lowercase letters (P < 0.05). The number of analyzed nuclei per genotype is indicated below boxplots. DAPI-stained nuclei extracted from rosette leaves of the indicated genotypes (right). Scale bar: 5 μm