Fig. 4

CHG and H3K9 hypermethylation in pol2a mutants. a Average methylation rates in CG, CHG and CHH contexts in L5 and pol2a-12. b Methylation rates in CG, CHG, and CHH contexts in L5, pol2a-12, Col-0 (WT), pol2a-8, and pol2a-10, averaged over non-overlapping 100 kb bins on chromosome 4. c Metaplots showing methylation levels at TEs upregulated in pol2a-12. Annotations were aligned to their 5′ or 3′ end and average methylation was calculated for each 100-bp bin from 3 kb upstream to 3 kb downstream. d Differentially methylated regions (DMRs) identified in pol2a-12 (see the “Methods” section). DMRs were further sorted between euchromatin and heterochromatin based on their genomic location. e Metaplots showing H3K9me2 enrichment (log₂ signal over input) in L5 and pol2a-12 at H3K9me2 peaks. Shaded areas show standard deviation. One replicate is shown