Skip to main content
Fig. 7 | Genome Biology

Fig. 7

From: RNA editing in cancer impacts mRNA abundance in immune response pathways

Fig. 7

ILF3 regulates PKR mRNA abundance and EMT in A549 cells. a Western blot confirming shRNA-mediated ILF3 KD in A549 cells (left). ILF3 mRNA levels (mean ± SD) were quantified in A549 shCtrl and ILF3 KD cells by qRT-PCR (right). ILF3 mRNA expression was normalized against gene TBP mRNA expression. Three biological replicates were performed. p value calculated via t-test, ****p < 0.0001. b Normalized mCherry expression (mean ± SD) for nonedited or edited versions of sites in the 3′ UTR of PKR in shCtrl or ILF3 KD A549 cells. Five biological replicates were performed. Normalized expression values were compared between edited and nonedited versions by two-sided t-test. *p < 0.05, **p < 0.01, n.s., not significant. c Images of A549 cells transfected with siRNAs targeting ILF3 (two different siRNAs were used to KD ILF3, siILF3_1, and siILF3_2) or control siRNAs (siControl). Scale bars: 100 μm. d Western blot detecting protein levels of ILF3, E-Cadherin, N-Cadherin, and internal control β-Actin in the siControl, siILF3_1, and siILF3_2 A549 cells. Three biological replicates were carried out for each experiment. e Normalized mRNA expression levels (mean ± SD) for ILF3, E-Cadherin, and N-Cadherin in the siControl, siILF3_1, and siILF3_2 A549 cells. Three biological replicates were carried out for each experiment. The expression values were compared between siILF3 and siControl via t-test. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not significant

Back to article page