Fig. 5

Identification and functional characterization of the candidate fruit shape gene. a A 1.67-Mb inversion highly associated with fruit shape. Top, Manhattan plot of GWAS of fruit shape. Bottom, the inversion supported by read alignments. Black arrows connected with solid lines represent normal paired-end reads mapped against the peach genome and orange arrows connected with dotted lines indicate split reads mapped against the breakpoints of the inversion. b Relative expression levels of PpOFP1 (Prupe.6G290900) during early fruit development in normal flat (ZH), aborted flat (XJ2) and round peach (HY). c Relative expression levels of PpOFP1 in transgenic tomato lines determined by qRT-PCR. d Phenotypes of transgenic Micro-Tom tomato lines overexpressing peach PpOFP1 gene. Scale bar = 5 cm. e Phenotypes of flower and fruit collected from wild type (WT) and overexpressing (OE) transgenic tomato. Scale bar = 0.5 cm. f Paraffin sections of flowers (top) and fruit 10 days after fertilization (bottom) in WT and OE transgenic tomato. Scale bar = 500 μm. g Cell number along the vertical axis of WT and OE tomato fruit at 10 days after fertilization