Fig. 1

Photograph and schematic drawing of tree 13 and tree 14. This wild P. trichocarpa, located near Mt. Hood, Oregon, experienced a decapitation event ~ 300 years ago. Tree 14 re-sprouted from the stump and ~ 80–100 years later tree 13 re-sprouted. a Leaf samples were collected from the labeled terminal branches. b Age was estimated for both the end of the branch (black font) and where it meets the main stem (gray italics). Ages with * indicate age was estimated using diameter; all other estimates were from core samples. Leaf samples of each branch was used to create genomic sequencing libraries, PacBio libraries, whole-genome bisulfite sequencing libraries, and mRNA-sequencing libraries