TY - JOUR AU - Dong, Xiaofeng AU - Munoz-Basagoiti, Jordana AU - Rickett, Natasha Y. AU - Pollakis, Georgios AU - Paxton, William A. AU - Günther, Stephan AU - Kerber, Romy AU - Ng, Lisa F. P. AU - Elmore, Michael J. AU - Magassouba, N’faly AU - Carroll, Miles W. AU - Matthews, David A. AU - Hiscox, Julian A. PY - 2020 DA - 2020/09/07 TI - Variation around the dominant viral genome sequence contributes to viral load and outcome in patients with Ebola virus disease JO - Genome Biology SP - 238 VL - 21 IS - 1 AB - Viral load is a major contributor to outcome in patients with Ebola virus disease (EVD), with high values leading to a fatal outcome. Evidence from the 2013–2016 Ebola virus (EBOV) outbreak indicated that different genotypes of the virus can have different phenotypes in patients. Additionally, due to the error-prone nature of viral RNA synthesis in an individual patient, the EBOV genome exists around a dominant viral genome sequence. The minor variants within a patient may contribute to the overall phenotype in terms of viral protein function. To investigate the effects of these minor variants, blood samples from patients with acute EVD were deeply sequenced. SN - 1474-760X UR - https://doi.org/10.1186/s13059-020-02148-3 DO - 10.1186/s13059-020-02148-3 ID - Dong2020 ER -