Fig. 3
Effect of the novel alleles on translation of the downstream primary open reading frame. a The novel alleles generated in the T0 generation. Nucleotide substitutions and small deletions are indicated in red. The amino acid residues of SC-uORF corresponding to the codons in the DNA sequence are indicated by yellow filled boxes. Changed amino acids are indicated by red filled boxes. b Schematic of the dual-luciferase reporter vector. 35s pro, cauliflower mosaic virus 35s promoter. REN, Renilla reniformis luciferase. LUC, luciferase. c Effect of the novel alleles on translation of the pORF in the dual-luciferase reporter system. d Effect of the novel alleles on transcription of the pORF in the dual-luciferase reporter system. In c and d, mean values (±SD) are compared to those for wild-type plants using Student’s t tests, **P < 0.01. AL#, allele #