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Table 5 Novelty of CAZyme proteins

From: 1200 high-quality metagenome-assembled genomes from the rumen of African cattle and their relevance in the context of sub-optimal feeding

Protein set

PID (% protein identity) thresholds

100%

99%

95%

90%

85%

80%

nr

9

93

402

960

2020

3653

env_nr

1

1

11

33

210

661

M5nr

1

2

96

532

1764

4073

UniProt/TrEMBL

2

8

83

415

1169

2468

Hess

839

1553

5404

10,947

17,597

24,151

RUG1

116

665

3058

7173

12,489

18,590

RUG2

338

2028

8243

17,342

26,825

35,159

Total unique

1286

4172

15,012

28,561

40,458

49,323

  1. Counts of hits at distinct minimum percentages of protein identity (PID) thresholds (100%, 99%, 95%, 90%, 85% and 80%) of all 68,850 carbohydrate active proteins from African MAGs against nr, env_nr, M5nr and UniProt/TrEMBL databases and the Hess et al. (Hess), original RUG (RUG1) and RUG 2.0 (RUG2) predicted proteins. The total unique number represents the number of proteins from the African MAGs that have a hit in at least one database