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Table 1 Summary of individual sequencing runs

From: Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs

Gene Mutant allele frequency (%) / Type Amplicon size Sequencing platform Read count Reads with UMI UMI groups for variant calling
(≥ 5 reads)
Median read number per UMI group UMI groups with introduced mutation Somatic SNV count Somatic SNV load per megabase SV groups
EPOR 1:100 (1%) 168 bp Nanopore 17,634 6444 284 7 2 (0.7%) 0 N/A N/A
EPOR 1:1000 (0.1%) 6789 bp PacBio 227,206 136,399 3184 6 4 (0.126%) 192 9.0 3
EPOR 1:10,000 (0.01%) 168 bp Nanopore 1,093,683 494,009 15,598 8 1 (0.006%) 10 7.1 N/A
EPOR 1:10,000 (0.01%) 168 bp Illumina 7,488,257 7,236,007 132,341 7 5 (0.004%) 85 7.1 N/A
PANX1 (Pan1) WT 7077 bp Nanopore 576,583 165,628 2810 6 N/A 73 3.8 0
PANX1 (Pan3) WT 6595 bp Nanopore 389,726 133,215 3867 7 N/A 103 4.1 0
PANX1 (Pan1) Cas9 editing 7077 bp Nanopore 2,761,805 613,147 3479 7 N/A 275 11.3 189 (5.4%)
PANX1 (Pan3) Cas9 editing 6595 bp Nanopore 3,078,165 1,042,582 7281 10 N/A 624 13.1 204 (2.8%)