Fig. 1

Identification of transcriptional regulatory networks of mesenchymal-associated tumor-associated macrophages (MA-TAMs). a Heatmap representation of MA-TAM encoding genes in TAMs that were isolated from three mesenchymal (MA-TAMs) and six non-mesenchymal (non-MA-TAMs) tumor specimens. b Gene Set Enrichment Analysis (GSEA) of PPARG, BATF, and SPI1 in MA-TAM compared to non-MA-TAM (upper panel). Global regulatory networks between MA-TAM master regulators (blue) and their target genes (red) (bottom panel). Positive and negative interactions between master regulators and their target genes are represented in green and red, respectively. Master regulators with P < 0.05 are highlighted in boldfaced blue. c Scatter plot correlation between MA-TAM signature score and master regulator activity in 91 longitudinal pair samples. Recurrent tumors were categorized into two groups: mesenchymal and non-mesenchymal. d Multiplex immunohistochemical analysis of glioblastoma specimens classified as either mesenchymal or non-mesenchymal. The co-localization of CD68, MARCO, BATF, PPARG, SPI1, and YKL40 are indicated with white arrows