Fig. 6

SERBP1 knockdown increased expression of genes linked to neurogenesis and nervous system development. a Enriched Gene Ontology terms related to genes upregulated upon SERBP1 knockdown in U251 cells. Gene set was analyzed using Panther [24] and GO terms were compiled using [25]. Most representative terms associated with nervous system development and function are listed. b Network analysis of genes implicated in neuronal differentiation affected by SERBP1 knockdown. The network was built using String [26] considering interaction (experimental evidence), text mining, and co-occurrence. Different colors were used to indicate clusters. c Heatmap shows that genes upregulated upon SERBP1 knockdown cells display increased expression during murine neurogenesis—0 day/stem vs. 4 days/differentiated cells. d qRT-PCR shows SERBP1 and β-III Tubulin expression in neuronal stem cells (NSCs) and differentiated cells. e Genes upregulated upon SERBP1 knockdown showing decreased expression in GBM in reference to LGG are labeled in blue, genes that show reduced expression in GBM in reference to normal brain (cortex) are labeled in green and genes that are methylated (H3K27me3) in GBM cells [45] are labeled in orange. f Western blot showing that SERBP1 knockdown leads to a decrease in H3K27me3 in GBM cells