Fig. 4

Conserved boundaries show a gradient of enrichment of Hi-C features. a Schematic representation of the boundary consensus algorithm using an example of three cell types and a total of ten TADs that yields eight boundaries with conservation scores 1 to 3. For each conservation score, we report b the number and c the size distribution obtained when starting with the seven primary blood cell type TAD datasets of Javierre et al. [12]. d For each conservation score, the number (top) and proportion (bottom) of consensus boundaries that intersect a GM12878 cell boundary are shown. e Average number of PC-HIC loops from Javierre et al. [12] that span consensus boundaries of each conservation score. At the centre, the real boundary set is shown and then the coordinates are shifted to the left and right in steps of 10 kb up to 400 kb. f Density of consensus boundaries conserved in at least two cell types (s = > 2) on Genomic Regulatory Blocks (GRB) obtained from Harmston et al. [41], aligned at the centre of 5-Mb regions and ordered from largest to smallest. g Average directionality index in 5-kb windows, computed on GM12878 and projected onto the s1 to s7 boundaries in 1-Mb windows