Fig. 4

PiggyBac transposition identifies 281 known and novel driver genes cooperating with mutant EGFR in brain and spinal gliomas. a Oncoprint showing the top CIS genes across all 96 brain and spinal gliomas (Bonferroni adjusted p < 0.01 for each gene, Gaussian kernel convolution analysis). “IS,” total number of insertion sites; “% tum.,” percentage of tumors with an insertion in corresponding gene. The most well-known brain glioma tumor suppressors are among the top 4 genes (Cdkn2a, Nf1, and Pten). Novel glioma genes include Sox6, Spred1, and Tcf12. b The position of all transposon insertions across Nf1 (a known brain tumor driver) in brain and spinal gliomas, showing a gene-disruption insertion pattern. Blue arrow = antisense orientation; red arrow = sense orientation with respect to gene direction. c. Novel glioma drivers, Sox6, Spred1, and Tcf12 also have disruptive insertional patterns. These figures show all piggyBac insertions in brain tumors