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Table 7 Percentages of the 17 cell types in both PBMC-1 and the validation dataset. All phony-type GEM clusters identified in PBMC-1 have close-to-zero presence in the validation dataset, suggesting that these cell types do not really exist and are artifacts of multiplets

From: GMM-Demux: sample demultiplexing, multiplet detection, experiment planning, and novel cell-type verification in single cell sequencing

Cell type

PBMC-1 (%)

Validation PBMC (%)

CD19+ (B cells)

2.72

5.31

CD3+CD4+ (helper T cells)

37.84

40.03

CD3+CD8+ (cytotoxic T cells)

12.81

16.22

CD14+CD16- (classical monocytes)

12.85

8.79

CD14+CD16+ (non-classical monocytes)

1.79

0.76

CD56+CD16- (CD16- NK cells)

0.84

1.06

CD56+CD16+ (CD16+ NK cells)

8.41

14.60

CD11+CD14-CD16- (DCs)

1.16

1.06

CD14+CD56+

0.42

0.08

CD3+CD4+CD14+

2.93

0.00

CD3+CD4+CD19+

0.68

0.08

CD3+CD4+CD56+

1.81

0.00

CD3+CD4+CD8+

2.99

0.42

CD3+CD8+CD14+

0.93

0.15

CD3+CD8+CD19+

0.27

0.12

CD3+CD8+CD56+

0.35

0.08

CD3+CD4+CD14+CD56+

0.08

0.00