TY - JOUR AU - Ji, Zhicheng AU - Zhou, Weiqiang AU - Hou, Wenpin AU - Ji, Hongkai PY - 2020 DA - 2020/07/03 TI - Single-cell ATAC-seq signal extraction and enhancement with SCATE JO - Genome Biology SP - 161 VL - 21 IS - 1 AB - Single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) is the state-of-the-art technology for analyzing genome-wide regulatory landscapes in single cells. Single-cell ATAC-seq data are sparse and noisy, and analyzing such data is challenging. Existing computational methods cannot accurately reconstruct activities of individual cis-regulatory elements (CREs) in individual cells or rare cell subpopulations. We present a new statistical framework, SCATE, that adaptively integrates information from co-activated CREs, similar cells, and publicly available regulome data to substantially increase the accuracy for estimating activities of individual CREs. We demonstrate that SCATE can be used to better reconstruct the regulatory landscape of a heterogeneous sample. SN - 1474-760X UR - https://doi.org/10.1186/s13059-020-02075-3 DO - 10.1186/s13059-020-02075-3 ID - Ji2020 ER -