Skip to main content
Fig. 6 | Genome Biology

Fig. 6

From: Mapping regulatory variants controlling gene expression in drought response and tolerance in maize

Fig. 6

Validation of abh2 local variants affecting the gene expression and the negative association of abh2 with drought tolerance. aabh2-based association mapping and pairwise linkage disequilibrium (LD) analysis for the variants of P < 10−6. The lead SNP of the abh2 local eQTL (SNP 2334) in the 3′-UTR and newly identified variants (P < 10−10) are connected to the pairwise LD diagram with solid lines. Asterisks indicate the LD between these variants. b Six haplotypes of abh2 were defined by the variants (P < 10−6). Gene expression levels of each haplotype group are displayed in the box plot, and the accession number of each haplotype was labeled in the left. Yellow color indicates the genotype of Haplotype1, and the blue color indicates the counterparts. c DNA sequence alignment of abh2-CRISPR locus of three homozygous lines. d Drought test of three abh2-CRISPR lines. Photographs were taken under well-watered conditions and after the stress treatment. Eighteen plants were tested for each genotype in each experiment. Statistical data were obtained from four replicates. e Rate of leaf water loss. Six leaves of each genotype were measured in each experiment, with three replicates. f ABA content quantification, with three replicates. Error bars, s.d.; statistical significance was determined by a two-sided t test: *P < 0.05, **P < 0.01

Back to article page