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Fig. 3 | Genome Biology

Fig. 3

From: Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

Fig. 3

Sorting and functional validation of distinct populations. a Immunofluorescence staining showing expression of CDH5, CD44, and CD43 on haematopoietic clusters of cells at EHT D5. Scale bar is 50 μm. b Flow cytometry analysis showing the sorting strategy for six populations (P1–P6). Results are representative of at least 3 independent experiments, using 2 distinct hPSC lines. c CFU assay showing the differentiation potential of cells isolated from P1 to P6. The ability to produce multilineage CFU-GEMM colonies is compared to the unsorted population. Data are mean ± SEM of 4 independent experiments, with statistical significance compared to control as *P < 0.5, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by one-way ANOVA. Degrees of freedom DFn = 6, DFd = 21, value F = 15.21

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