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Fig. 1 | Genome Biology

Fig. 1

From: Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

Fig. 1

Culture system for modelling EHT in vitro. a Schematic of the differentiation protocol used to model EHT with hPSCs. D, day. b Representative images of cells progressing through EHT. Scale bar is 400 μm. c Quantification of colonies generated in a CFU assay using cells collected at EHT D5. Data represent means ± SEM of 3 independent experiments. BFU-E, burst forming unit-erythroid; CFU-E, colony forming unit-erythroid; CFU-GM, colony forming unit-granulocyte-macrophage; CFU-G, colony forming unit-granulocyte; CFU-M, colony forming unit-macrophage; CFU-GEMM, colony forming unit-granulocyte-erythrocyte-macrophage-megakaryocyte. d, e Flow cytometry analysis of d cells at D8/EHT D0 and e during EHT culture at multiple time points. Results are representative of at least 3 independent experiments

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