SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

Table 1 Mutation frequencies induced by SWISSv3 in T₀ rice plants

Types of genome editing	Target sites	No. of transgenic rice lines	No. of mutants^a	Genotype of mutations^b	Heterozygous/homozygous
CBE	OsALS-T2	55	14 (25.5%)	C₆>T₆ (7); C₆>G₆ (2); C₆C₇>T₆T₇ (4); C₆C₇C₁₆>T₆T₇T₁₆ (1)	14/0
ABE	OsACC-T2		9 (16.4%)	A₄>G₄ (7); A₇>G₇ (2)	9/0
Indels	OsBADH2-Indels		29 (52.7%)	deletions (22); insertions (7)	19/10

^aBased on the number of T₀ lines (rice) carrying the observed mutations over the total number of T₀ transgenic rice lines analyzed
^bThe genotypes of indels were analyzed with the online tools DSDecodeM and TIDE (see the “Methods” section)

ISSN: 1474-760X