Fig. 5

Interactions between evolutionarily conserved ISGs modulate the transcriptional response to IFN stimulation. a Left, distribution of correlations between pairs of proteins from core, conserved, and human-specific ISG sets in PCP chromatograms. Right, negative base-10 logarithm of p values from Brunner–Munzel tests of the difference in medians. b Schematic overview of the affinity purification–mass spectrometry experiments of specific core ISGs in IFN-stimulated or unstimulated cells. c Gene set enrichment analysis barcode plots [56], showing ranks of core, conserved, and human-specific ISG products in comparisons of immunoprecipitations of IFI35 (left), NMI (middle), and STAT1 (right) from IFN-stimulated or unstimulated cells, alongside negative base-10 logarithms of p values for each ISG set. d PCP chromatograms from a representative replicate of IFI35, NMI, and STAT1 in IFN-stimulated and unstimulated cells. e Protein domain content of IFI35 (top) and STAT1 (bottom). f Top, luciferase activities from cells transfected with a 5 × ISRE-Fluc reporter construct to monitor STAT1 transcriptional activity (± SD). Cells were transfected with equal amounts of DNA in each case. Bottom, western blots of HA-tagged IFI35 expression from lysates transfected with reporter constructs. *p < 0.05. g Immunofluorescence micrographs of cells mock transfected or transfected with HA-tagged IFI35 expression constructs. Shown is a representative image. Scale bar = 10 μm