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Fig. 3 | Genome Biology

Fig. 3

From: Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

Fig. 3

Cell preservation protocol performance in cold-dissociated samples. a Cell type composition of freshly profiled and preserved cold-dissociated samples. b Number of differentially expressed genes (DEGs) detected between preserved and freshly profiled aliquots. Seurat Wilcoxon test [30] with logFC = 1, min detection rate 0.5, FDR < 0.05 as thresholds. c Expression and detection rates of DEGs with higher expression in cryopreserved samples in at least two cell types. d Expression and detection rates of DEGs with higher expression in methanol-fixed samples in at least nine cell types. aLOH: ascending loop of Henle; CD_IC: intercalated cells of collecting duct; CD_PC: principal cells of collecting duct; CNT: connecting tubule; DCT: distal convoluted tubule; PT: proximal tubule

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Genome Biology

ISSN: 1474-760X

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