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Fig. 4 | Genome Biology

Fig. 4

From: instaGRAAL: chromosome-level quality scaffolding of genomes using a proximity ligation-based scaffolder

Fig. 4

Step-by-step correction procedure. Correction procedure (top to bottom): (i) in silico restriction of the genome and binning, yielding a set of bins; (ii) reordering of all bins into scaffolds without taking into account their input contig of origin; typically, groups of bins from the same input contig naturally aggregate, but some bins get scattered to other scaffolds (e.g., bin 13, pink arrow), while others will be “flipped” with respect to the original assembly (e.g., bin 4, red arrows); (iii) reconstruction of the original input contigs by relocating scattered bins next to the biggest bin group; and (iv) bins in the original input contigs are oriented according to their original consensus orientation

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