Fig. 3

APEC constructed a differentiation pathway from scATAC-seq data from human hematopoietic cells. a The pseudotime trajectory construction scheme based on the accesson matrix and Monocle. b Principal component analysis (PCA) of the accesson matrix for human hematopoietic cells. The first principal component is not shown here because it was highly correlated with sequencing depth. HSC, hematopoietic stem cell; MPP, multipotent progenitor; LMPP, lymphoid-primed multipotential progenitor; CMP, common myeloid progenitor; CLP, common lymphoid progenitor; pDC, plasmacytoid dendritic cell; GMP, granulocyte-macrophage progenitor; MEP, megakaryocyte-erythroid progenitor. c Pseudotime trajectory for the same data constructed by applying Monocle on the accesson matrix. Paths A and B represent different pathways for GMP cell differentiation. d The deviations of significant differential motifs (HOXA9, GATA1, CEBPB, and TCF4) plotted on the pseudotime trajectory. e Distributions of the CMP sub-clusters on the trajectory. f Modified schematic of human hematopoietic differentiation