Fig. 2

Solutions to correct or prevent sampling time-induced artifacts. a tSNEs displaying the effect of varying processing times on the transcriptome profiles of 7378 PBMC before (left) and after (right) regressing out the time score for every highly variable gene. b kBET acceptance score distribution across sampling times with or without the computational correction. c tSNE showing the effect of PBMC culturing and activation with anti-CD3 Dynabeads over 2 days. d kBET acceptance score distribution across cell types with or without cell culture/activation. e tSNE highlighting the sampling effect between cells cryopreserved immediately (fresh, 0 h) or after 24 h and 48 h stored cold (4 °C) or at RT (21 °C). f kBET acceptance score distribution across storage temperatures