Fig. 7

tappAS analysis results and experimental validation AltTEM processing of Ctnnd1/p120. a Protein-level visualization of tappAS functional annotation for Ctnnd/p120. Exclusion of an exon in two of its isoforms causes an NLS motif to appear in the protein sequence. b Gene-, transcript-, and CDS-level expression of Ctnnd1. The gene is significant for both DIU and DCU, with major isoform switching of the nuclear isoforms (yellow and red) in OPCs. c DFI analysis results for the NLS motif in Ctnnd1. NLS inclusion is favored in OPCs. d Western blot analysis of Ctnnd1 protein p120 in nuclear cytosolic, cytosolic, and total fractions of NPCs and OPCs. An increase of the nuclear expression of the protein is observed in OPCs due to differential inclusion of the NLS, while cytosolic expression remains constant