Fig. 6

The impact of fusion proteins on chromatin organization. a Snapshot of Hi-C data from Juicebox corresponding to Chr 8: 63,616,214-69,456,200 at 10 kb resolution. Cells harboring CTCF, CTCFL, CLC, and LCL transgenes were treated with ID as indicated for 4 days. The corresponding FLAG ChIPs are shown on the x- and y-axis. b Subtraction heatmaps of Hi-C data from Juicebox corresponding to CTCF ID – CTCFL ID, CTCF ID – CLC ID, and CTCF ID – LCL ID. CTCF binding sites (CTCF ID: Flag ChIP) are shown on the y-axis and Flag ChIPs of CTCFL ID, CLC ID, and LCL ID on the x-axis, as indicated. c Insulation scores in boundaries of CTCF+CTCFL overlapping sites in CTCF depleted cells (CTCF I) as well as cells depleted of CTCF but induced to express transgenic CTCF, CTCFL, CLC, or LCL (ID condition). d Snapshot of Hi-C data from Juicebox showing partial recue of TADs when CLC and LCL were expressed in the absence of endogenous CTCF. The corresponding FLAG ChIPs are shown on the x- and y-axis. e Co-IP experiments showing interaction of RAD21 with transgenic CLC or LCL in the presence (D) and absence (ID) of CTCF