Fig. 2

a The Lung Tumor Microenvironment Interactome (LTMI) integrates data generated in this study, the FANTOM5 resource of ligand-receptor pairs, and PRECOG for prognostic associations of genes in bulk tumor samples. b Potential complexity of inter-cell-type signaling via secreted factors. Ligands or receptors were defined as significantly expressed in a cell type if they had TPM > 10, as in the FANTOM5 study. c, d Potential cross-talk between cell types in adenocarcinoma (c) and squamous cell carcinoma (d). Shown are the number of ligand-receptor pairs where each is a uniquely differentially expressed gene (uDEG) in the indicated cell type. Arrows X->Y indicate that the ligand is a uDEG in cell type X, while the corresponding receptor is a uDEG in cell type Y. e ANGPT1 and ANGPT2 compete antagonistically for receptor binding and have opposite prognostic associations in NSCLC. They are expressed on fibroblasts and endothelial cells respectively, with expression of their known receptors being predominantly in endothelial cells. (f) Expression patterns of ligands and receptors pair that are highly expressed (TPM > 10) in single-cell types (corresponding to the 1–1 entries for adenocarcinoma and SCC in panel (b). Pink indicates ligand whereas blue indicates receptor. Three rightmost panels are expanded views of the groups indicated in first panel