Fig. 5

Landscape of individual initiation events. a Empirical cumulative distribution function (ECDF) of the distances between cluster median points, grouped according to cluster dimension, and the closest known origin (ORI, confirmed, likely and dubious origins from OriDB [23]) center. Black, random elements; red, sporadic initiation events; light, median, and dark green, initiation clusters of dimension d = 2–4, d = 5–12, and d > 12, respectively. The number of elements in each category is written next to each curve. b Averaged RFD profile for the clusters grouped according to their dimension (− 10 kb, + 10 kb from the cluster median). rDNA cluster was excluded. c Density distribution of normalized replication timing (0 = start and 1 = end of S phase) for the whole genome (black) and the clustered (green) and sporadic (red) initiation events. d ACS-motif containing fraction of the indicated elements: randomized initiation events; confirmed, likely and dubious origins from OriDB [23]; sporadic (d = 1) and clustered (d = 2–4; d = 5–12; d > 12) initiation events. For randomized elements, the randomization was repeated 1000 times and the median and [0.01,0.99] percentile interval are shown (red bar). e–f ECDF of the distance between sporadic (red), clustered (green), and randomly shuffled (black) initiation events, and the center of the closest ORC [25] (e) or Mcm2-7 [27] (f) binding site