Fig. 4
From: Developmental regulation of canonical and small ORF translation from mRNAs
Regulated translation during embryogenesis. a Number of transcribed, ribosome-bound, and translated ORFs per class across Drosophila melanogaster embryonic development, showing stage-specificity in ON/OFF translation patterns. b Changes in average translation efficiency, per ORF class, across analyzed developmental time-points. cZ-score ratio analysis of translation efficiencies (TE) during successive developmental stages pinpointing ORFs subjected to significant developmental modulations of translation across ORF classes. d Number of developmentally regulated canonical ORFs subjected to downregulation (bottom, Z-ratio ≤ − 1.5) or upregulation (top, Z-ratio ≥ 1.5), per developmental transition. e Metagene plot for codon framing of 32-nt ribosome footprints across reproducibly “ribosome-bound-only” uORFs, showing a lack of three-nucleotide periodicity (framing) similar to that of mRNA-Seq (compared with translated uORFs in Additional file 1: Fig. S3C). f Distribution of log2(RPKMFP) values of ribosome-bound-only uORFs (red) and translated (framed) uORFs (green). Median nucleotide sizes of each pool are indicated above