Fig. 2

LADs are dynamic and heterogeneous domains. a Three classes of promoters identified in LADs: “repressed promoters” become active when experimentally removed from their LAD context, “escaper promoters” are active even when in a LAD, and “inactive promoters” are inactive regardless of their LAD context [80]. b Enrichment profiles of indicated genomic features in LAD borders. c Deletion of a LAD border within the Tcrb locus spatially reorganizes the locus [70]. When inactive, the locus contains a LAD border that separates repressed Vβ genes from the H3K27 acetylated recombination center containing active D and J genes. Deletion of the LAD border elicits spreading of H3K27ac into the LAD, activation of a set of Vβ genes, and looping of the active Vβ genes and the recombination center, favoring VDJ recombination. d A subset of LADs in mouse liver displays periodic patterns of association with the nuclear lamina [78]. LAD borders, rather than entire LADs, are most often affected