Fig. 5

Chromatin compaction and dynamics do not spatially correlate. a Spatial classification of signal intensity into euchromatin and heterochromatin [35] overlaid on an exemplary fluorescence image for quiescent (left) and stimulated (right) cells. b Average flow magnitude and c diffusion constant (n = 12) in euchromatin and heterochromatin for starved (left) and serum-stimulated cells (right). Statistical significance assessed by a two-sample t-test. d Overlay with the diffusion populations found by Hi-D. Black solid line corresponds to eu-/heterochromatin region boundaries. e Diffusion populations show a similar distribution over hetero- and euchromatin. The colors refer to the slow, intermediate, and high population respectively and each point corresponds to one nucleus. Statistical significance assessed by a two-sample t-test (*p < 0.05, **p < 0.01, ***p < 0.001). f–g Anomalous exponent as d–e. h Spatial autocorrelation at euchromatin (green) and heterochromatin (purple) of the flow magnitude between all accessible time lags in quiescent and serum-stimulated cells