Fig. 3

An in vitro reference experiment. a A reference lineage tracing dataset was generated using the technology proposed in Chan et al. [10] to human cells cultured in vitro for ∼15 generations. A total of 34,557 cells were analyzed after filtering and error correction. Only the initial split (into two plates) is shown. Analysis of the subsequent split (into four plates) is provided in Additional file 1: Fig S22. b–f Summary of relevant lineage tracing parameters for each clonal population in the experiment: b the number of characters per clone, c the number of states per target site, d the estimated mutation rate per target site, e the median dropout per target site, and f the proportion of uniquely marked cells. Gray shading denotes parameter regimes tested in simulations, and red-dashed lines denote the default values for each synthetic benchmarks