Skip to main content
Fig. 5 | Genome Biology

Fig. 5

From: CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma

Fig. 5

lncGRS-1 function is glioma specific. a RT-qPCR of lncGRS-1 transcript levels following ASO-mediated knockdown in NHA cells (n = 2 biological replicates per condition; error bars = SD). b Cell propagation time course in NHA cells with ASO-mediated knockdown of lncGRS-1. ASOs were re-transfected at day 7 (n = 2 biological replicates per condition; error bars = SD). c Left, fluorescence viability assay of malignant tumor cells and NHA cells following ASO-mediated lncGRS-1 knockdown. Right, apoptosis assay of malignant tumor cells and NHA cells following ASO-mediated lncGRS-1 knockdown (n = 2 biological replicates per condition; error bars = SD). d Cell cycle phase analysis following lncGRS-1 knockdown in GBM U87 using flow cytometry. e RNA-seq differential gene expression analysis of lncGRS-1 knockdown in GBM U87 (left), DIPG SF8628 (middle), and NHA (right) cells using lncGRS-1 ASO #1 and ASO #2 as biological replicates, compared to negative control ASO, 24 h following transfection (n = 2 biological replicate cultures per ASO condition). Green, genes adj. p value < 0.05. Red triangle, lncGRS-1

Back to article page