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Fig. 3 | Genome Biology

Fig. 3

From: CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma

Fig. 3

lncGRS-1 is a primate-conserved radiation sensitizer target in glioma. a Nanopore direct RNA-seq spliced reads aligned to the lncGRS-1 gene body in U87 cells, with GENCODE v29 transcript models of lncGRS-1 (CTC-338 M12.4) and multiz alignment for conservation. b Subcellular fractionation followed by qPCR of transcripts in U87 cells. c Single molecule FISH of lncGRS-1 in GBM SF10360 and DIPG SF8628 primary glioma cells. Scale bar = 5 μm. d RT-qPCR for lncGRS-1 after CRISPRi targeting in U87 (n = 2 biological replicates; error bar = SD). e, f Internally controlled growth assays of U87 cells with CRISPRi knockdown of lncGRS-1 in the absence (e) and presence (f) of fractionated radiation. g, h Cell propagation assay of purified populations of U87 cells with lncGRS-1 CRISPRi knockdown in the absence (g) and presence (h) of fractionated radiation. For eh, n = 2 biological replicates per condition; error bars = SD; two-tailed Student’s t test. N.S., not significant. Radiation delivery timepoints indicated below the x-axis

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