Fig. 6
From: Successful generation of epigenetic disease model mice by targeted demethylation of the epigenome
Postnatal development and phenotype of SRS modeling mice. a Appearance of vector-integrated and control female mice at 4 weeks of age. b Growth retardation was observed in vector-integrated mice. n indicates the number of mice used for the analysis. Error bars, mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t test). cH19-DMR methylation status analyzed by COBRA in liver at 8 weeks of age. Vector-integrated mice frequently showed demethylation. Error bars, mean ± s.d. *P < 0.05, **P < 0.01 (two-tailed Student’s t test). d Food intake (g/day) was examined in vector integrated and not integrated female mice (3 weeks of age). *P < 0.05 (two-tailed Student’s t test). e Glucose tolerance tests were performed after an overnight fast in vector integrated and not integrated male mice (7 weeks of age) fed normal chow. Blood glucose concentration appears to be lower in epigenome-edited mice. *P < 0.05 (one-tailed Student’s t test). Error bars, mean ± s.d. f Vector-integrated mice showed degeneration (2/3) and fibrosis (3/3) in cardiac muscle fiber whereas vector non-integrated mice did not show these phenotypes (0/3). HE, hematoxylin and eosin stain; MT, Masson’s trichrome stain. Scale bars: 50 μm