Fig. 2
From: Successful generation of epigenetic disease model mice by targeted demethylation of the epigenome
Generation of epigenome-edited (EpiEdit) mice via the tetraploid complementation method. a Schematic for generation of epigenome-edited ESC-derived mice. b Percentages of DNA methylation by COBRA in H19-DMR (m1–m4) and promoter (p1-p4) in undifferentiated ESCs (B6/J) at day 5 after transfection. Error bars, mean ± s.d. (biological triplicate). c Percentages of DNA methylation by COBRA in H19-DMR (m2) in undifferentiated ESCs (B6x129) during in vitro culture. d, days of culture after transfection. Error bars, mean ± s.d. (experimental triplicate). d Epigenome-edited newborn mice showed significant DNA demethylation at H19-DMR (m1–m4) and promoter (p1-p4) by COBRA. e Expression analysis by qPCR. Epigenome-edited ESC-derived newborn mice showed upregulation of H19 and downregulation of Igf2 expression compared to control mice. f Epigenome-edited ESC-derived newborn mice did not show growth retardation. n indicates the number of mice used for the analysis. Error bars, mean ± s.d. * P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant (two-tailed Student’s t test)