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Fig. 3 | Genome Biology

Fig. 3

From: A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing

Fig. 3

The stringency of CeCas12a, LbCas12a, and AsCas12a for recognizing canonical TTTV and non-canonical C-containing PAMs in human cells. a In vivo cleavage activities of Lb, As, and Ce toward different PAMs. Indel frequencies for 82 endogenous target sites with the different PAMs were measured in mammalian cells by deep sequencing. Percent modified indicates the percentage of reads containing indels compared with the wild-type sequence. b Stringency for recognizing canonical TTTV and non-canonical C-containing PAMs. The relative percent modified frequency was determined by the ratio of C-containing PAM to TTTV PAM. Data are shown as mean ± s.e.m. (n = 3); *P < 0.05, **P < 0.01, ***P < 0.001 (Mann-Whitney)

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