Fig. 4

Accurate identification of translocated chromosomal pairs by HiNT. a The distribution of the rank product scores for all chromosomal pairs in K562 before (left) and after (right) adjustment by background subtraction. Chromosomal pairs in pink and blue correspond to two FISH-validated translocation pairs (chr1, chr21) and (chr1, chr18); the one in yellow corresponds to a chromosome pair (chr16, chr19) without translocation. After matrix adjustment, the blue pair now has a lower score and the yellow pair has a higher score, as desired. TP: true positive, TN: true negative, FN: false negative, FP: false positive, 0.05 is used as the cutoff. b Receiver-operator characteristic (ROC) curves show HiNT performs better after the background subtraction. Areas under the ROC curves (AUCs) are shown in parentheses. c The original, background (average of multiple other Hi-C maps), and the adjusted maps are shown for the three cases highlighted in panel a. Validated translocations are marked by circles