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Fig. 6 | Genome Biology

Fig. 6

From: Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium

Fig. 6

Smarcad1-dependent Colitis susceptibility. a, b 1% DSS-induced colitis phenotype in WT and Smarcad1-KO animals with initial (non-enriched) microbiota (a) and enriched microbiota (b, 2 independent experiments shown: b-1/b-2). Experiments a and b-1 were terminated after 15 days (n = 5 for WT/KO), and experiment b-2 after 14 days with one mouse culled after 10 days due to extensive weight loss (n = 8 for WT, n = 6 for KO). SEM indicated by error bars. Indicated p values determined by 2-way ANOVA with Holm-Sidak’s multiple comparisons test, performed separately for each experiment. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Full statistical results are listed in Additional file 2: Table S1. c, d Intestinal colon epithelium localization (c) and quantitation (d) of MPO-positive cells (green, neutrophil/lymphocyte-marker) by IF staining and nuclear counterstaining with DAPI (blue) shown as maximum intensity projections. The 14-day colitis induction with enriched microbiota. Representative replicates, full image set, and negative staining controls shown in Additional file 1: Fig. S7. Scale bars, 40 μm. WT, wild type; KO, Vil-cre-mediated tissue-specific knockout of Smarcad1. d Epithelial and sub-epithelial number of MPO-positive cells per imaged area, quantified from the full image set shown in Additional file 1: Fig. S7 (3 biological replicates for WT/KO, 2–4 technical replicates each). Indicated p values determined by 2-way ANOVA with Holm-Sidak’s multiple comparisons test, performed separately for each epithelial/sub-epithelial localization. Full statistical results are listed in Additional file 2: Table S1

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