Fig. 3

Ultraplexing and classical molecular barcoding on a set of ten S. aureus isolates. For different read assignment methods applied to the same set of Nanopore reads, the distribution of contigs per assembly (a), the distribution of assembly lengths (b), the distribution of SNPs per assembly (c), and the distribution of reference recall (d) are shown. SNPs and reference recall were calculated relative to assemblies based on molecular barcoding, and the same Illumina sequencing data were used throughout. Barcoded, reads assigned according to molecular barcodes; Ultraplexing, reads assigned by the Ultraplexing algorithm; Random, reads assigned randomly