Fig. 6
Genomic-GC effect on ∆LFE in eukaryotes shows divergence in high GC-content species that is not observed in other domains, while low GC-content species have weak ∆LFE. a mean ∆LFE values for eukaryotes in the range 100–300 nt from CDS start, plotted against genomic-GC. Fungi are highlighted in blue. There is no linear relation between the variables (R2 = 0.01), but there is strong statistical dependence nevertheless (MIC = 0.582, p value < 2e−5, N = 78); see some explanation on MIC in the main text. b PCA plot for the same species shows the same two classes of ∆LFE profiles in eukaryotes having high genomic-GC (top and bottom regions; see “Visualization” under the “Methods” section for details). On the left, ∆LFE profiles are plotted in the positions given by their first 2 PCA components. On the right, genomic-GC values for the profiles plotted at the same coordinates. Short species names are listed in Additional file 1: Table S3