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Fig. 1 | Genome Biology

Fig. 1

From: Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS

Fig. 1

lrTAPS of 4 kb model DNA. a Schematic of lrTAPS for targeted long-read DNA methylation sequencing. b The upper panel (from top to bottom): methylation of a 4-kb model DNA obtained from bisulfite sequencing (short-read Illumina sequencing), Nano-TAPS, SMRT-TAPS, and native Nanopore methylation sequencing using Nanopolish or Tombo. The lower panel shows examples of individual long-reads from Nano-TAPS and SMRT-TAPS. The red bars indicate methylation. Black and purple bars indicate sequencing errors (deletions and insertions, respectively). c Scatter plots showing all pairwise correlations of all CpG sites among Nano-TAPS, SMRT-TAPS, native Nanopore methylation calling (Nanopolish and Tombo), and Bisulfite-seq, with correlation coefficient showing on top of each plot. d ROC curve and AUC comparing Nano-TAPS, SMRT-TAPS, and native Nanopore methylation sequencing (Nanopolish and Tombo), using DNA methylation from bisulfite sequencing (methylation level > 3% was designated as methylated) as the truth

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